Application

Research CategoryNeuroscience

Anti-Clock Antibody detects level of Clock & has been published & validated for use in ELISA & WB.

Western blot: 1-10 µg/mL using ECL.

ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well.

Optimal working dilutions must be determined by the end user.

Research Sub CategoryCircadian Rhythm & Sleep

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

An 18 amino acid peptide mapping downstream of the serine rich region of mouse Clock (Antoch et al. 1997; King et al. 1997).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS and 0.1% BSA.

Specificity

Recognizes mouse Clock. The immunogen shows no significant sequence homology with dPER or other known proteins.

SPECIES REACTIVITIES: The immunogen sequence is 92% conserved in rate and 90% in human. Reactivity with other species has not been confirmed.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.